Molecular detection of multi-drug resistance in Enterobacteriaceae

Dates and venue

Objectives

It is the specific intention of this training programme to disseminate and share the knowledge obtained on phenotypic and molecular detection and molecular characterization methods of strains harbouring SGI-1 with WP21 partners. Because the public health risk of epidemic clones, next to their virulence, is related to the potential acquisition of additional resistance determinants of relevance (ESBLs, plasmid mediated quinolone resistance, integrons, plasmids), detection and characterzation methods are included in the course. Moreover a nano-array developed for the rapid detection of resistance genes will be demonstrated.

Programme

The training course will be a mixture of lectures of highly qualified internationally well known researchers and practical training of methods used for analysis of MDR Enterobacteriaceae.

The training does not focus solely on SGI-1, but on all aspects of relevance when working with MDR Enterobacteriaceae. Therefore the topics will be: SGI-1, ESBLs, Plasmids, QNR, Integrons. On each topic top lecturers will be invited: SGI-1 Axel Cloeckaert (INRA), ESBLs Neil Woodford (HPA), Plasmids Alessandra Carattoli (ISS), QNR Laurent Poirel (Hospital de Bicetre, Paris), Integrons John Threlfall (HPA).

Practical training topics will include: classic PCR, Taqman and or Lightcycler RT-PCR, Sequencing, Array techniques, pyrosequencing.

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